Interactions of phage P22 tails with their cellular receptor, Salmonella O-antigen polysaccharide
نویسندگان
چکیده
منابع مشابه
Recombinational circularization of Salmonella phage P22 DNA.
The development of phage P22 following infection involves a mandatory recombination step, the timing of which is the same in both the lytic and lysogenic pathways. Covalently circular molecules of phage DNA were identified by alkaline sucrose sedimentation of infected cell lysates. The time of appearance of these structures corresponds to the time of the essential recombination step. On infecti...
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Classical identification of S. typhi involves culture on primary agar plates and requires another 24 hours for biochemical testing. The colonies suspected to be Salmonellae will be further tested with their speci fic antisera. Most isolates of Sal monella from natural sources can be classified serologically into five groups, A to E, by a combination of multiple o (somatic), H (flagella) and V...
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Phages are promising alternatives to antibodies as the biorecognition element in a variety of biosensing applications. In this study, a monolayer of bacteriophage P22 whose tailspike proteins specifically recognize Salmonella serotypes was covalently bound to glass substrates through a bifunctional cross linker 3-aminopropyltrimethoxysilane. The specific binding of Salmonella typhimurium to the...
متن کاملMutations that improve the ant promoter of Salmonella phage P22.
Mutations that increase the activity of the promoter for the antirepressor gene of phage P22 were isolated by pseudoreversion of four severe promoter-down mutations. The sequence changes in these pseudorevertants include single base pair substitutions, single base pair deletions, tandem double base pair deletions and multisite mutations. The single base pair substitutions change nonconsensus ba...
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A cosmid (pPR1347) carrying both the rfb gene cluster and the rfc gene of a Salmonella group B serovar has been constructed; Escherichia coli K-12 strains carrying this cosmid produce long-chain O antigen, are sensitive to phage P22, and can be transduced by P22. Some of the benefits of P22 transduction are now available for studying E. coli and potentially other genera.
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ژورنال
عنوان ژورنال: Biophysical Journal
سال: 1996
ISSN: 0006-3495
DOI: 10.1016/s0006-3495(96)79402-x